t4 dna ligase中文是什么意思
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用"t4 dna ligase"造句"t4 dna ligase"怎么读"t4 dna ligase" in a sentence
中文翻译手机版
- t4 dna连结酶
- t4dna连接酶
- "dna"中文翻译 DNA = 1.deoxyribonucle ...
- "ligase"中文翻译 n. 【生物学】联结酶。
- "t4 rna ligase" 中文翻译 : t4rna连接酶
- "dna ligase" 中文翻译 : dna连接酶,多脱氧核糖核苷酸合酶; dna黏合酶; 脱氧核糖核酸连接酶
- "t4 dna topoisomerase" 中文翻译 : t4 dna拓扑异构酶
- "t4 dna连结酶" 中文翻译 : t4 dna ligase
- "taq dna ligase taq dna" 中文翻译 : 连接酶
- "taq dna ligase" 中文翻译 : taq dna连接酶
- "t4 dna拓扑异构酶" 中文翻译 : t4 dna topoisomerase
- "t4" 中文翻译 : 环三甲撑三硝胺; 甲状腺素,四碘甲状腺原氨酸
- "ligase" 中文翻译 : n. 【生物学】联结酶。
- "l-t4" 中文翻译 : 优甲乐
- "t4 8w" 中文翻译 : 大量非标光源
- "t4 antigen" 中文翻译 : cd4抗原
- "t4 bacteriophage" 中文翻译 : t4噬菌体
- "t4 cell" 中文翻译 : t4细胞
- "t4 hyperthyroidism" 中文翻译 : t4甲状腺功能亢进症
- "t4 preparation" 中文翻译 : t4 制剂
- "t4 thyrotoxicosis" 中文翻译 : t4型甲状腺毒症
- "t4 uptake" 中文翻译 : 甲状腺素摄取量
- "t4 制剂" 中文翻译 : t4 preparation
- "t4细胞" 中文翻译 : t4 cell
- "tbp t4" 中文翻译 : 甲状腺素结合蛋白
- "tetraiodothyronine (t4)" 中文翻译 : 四碘甲腺原氨酸
- "amido ligase" 中文翻译 : 酰胺边接酶; 酰胺连接酶
例句与用法
- Then cdnas and puc18 vectors were linked by t4 dna ligase and transformed into e . coli strain dh5 - alpha to generate cdna library that size is 4 . 9 l06 recombinants
将cdna与载体连接,并导入dh5感受态细胞中,构建成cdna文库。 - 4 . the construction of middle - clone vector and expression vector the puc - cp and pgem - 7z plasmid were digested by kpnl and bamhi , and collected the digested cpti fragment and the pgem - 7z , then ligated by t4 dna ligase and formed the pgem - cp
中间载体及表达载体的构建将puc - cp质粒和pgem ? 7z质粒,用kpni和bamhi酶切,分别回收cpti片断和酶切后的载体片段,用t _ 4连接酶连接构建成中间载体pgem - cp 。 - Sub - - clone of s , . / hbsag fusion gene : pbuescripts , . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme , and were linked under t4 dna ligase , ppiczaa s , , / hbsag was constructed and transformed to e . coli
Hbsag质粒与ppiczaa载体分别经xhol和xbaln切,再在t4dna连接酶作用下进行连接,获得工程菌表达型ppiczaas ; hbsag质粒,转化大肠杆菌t0p10细胞,经xhol和xbal与sacll和xbal酶切电泳,证实s ; 。 - At first . then eight a - amylase gene fragments were cloned with the genomic dnas as templates by routine pcr . following that , these gene fragments and plasmid vectors , pbluescript ii ks + and puc18 , were cut by bamh i and kpn i . the prepared insert dna and vector dna were linked by t4 dna ligase
利用vectornti6 . 0软件,对所克隆的序列用相邻接点法( neighborjoining州j ) method )进行多序列比对,分析其同源性,并构建基因进化树。 - The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme , were linked by means of t4 dna ligase and transformed into e . coli jm109 permissive cells , yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid
将重组质粒pugedna与转移载体pfastbacldna用bamhi和ecori双酶切处理, t _ 4dna连接酶连接,用连接产物转化大肠杆菌jm109感受态细胞,得到重组转移载体质粒pfastbac - gedna 。 - A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a , yielding a 1 . 7kb band . the segment was linked to puc19 plasma dna by means of t4 dna ligase , transformed into e . coli jm109 permissive cells , and incubated on lb fray containg amp , x - gal and iptg . small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr , resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna连接酶使ge基因与经bamhi 、 kpni同样双酶切的puc19质粒dna连接;用连接产物转化大肠杆菌jml09感受态细胞,置含amp 、 x - gal和iptg的lb平板上培养12 20小时;挑取白色菌落于选择性培养基扩大培养,碱裂解法小量提取质粒dna ,并进行酶切分析鉴定,结果获得整合有prvge基因的重组质粒pugedna ,并与其它prv分离株进行ge基因序列同源性分析。 - First , the purified pezzis and pcr product of angiostatin are digested by ecor . i and xba i . after purifying the digested products respectively , we ligate these two kinds of dna by t4 dna ligase and construct the recombinant plasmid pezz18 - as . then transform it to the competent e . coli dh5a
用限制性内切酶ecori与xbai对目的基因as 、表达载体pezz18行双酶切,酶切产物纯化后利用大肠杆菌t _ 4dna连接酶连接构成重组子pezz18 - as ,并转化e . colidh5 ,经氨苄青霉素lb平板初筛后,以菌液pcr和重组子的单、双酶切行进一步鉴定。
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